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PROJECT TOPIC:  EFFECT OF METHANOLIC LEAF EXTRACT ON ACALYPHA WILKESIANA ON WEIGHT PARAMETERS IN PARACETAMOL INDUCED HEPATOXICITY IN MALE RATS
Department:  Bio Chemistry
AMOUNT:  10,000
FORMAT:   MS WORD
PAGES:  70
 
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2.1 MATERIALS                                        

2.1.1 PLANT MATERIALS FOR ANALYSIS

Fresh mature plants of Acalypha wilkesiana were collected from the natural habitat in October within Ambrose Alli University, Ekpoma, Edo State, Nigeria. The plant was authenticated in the Department of Botany, Ambrose Alli University, Ekpoma, Edo State, Nigeria. The leaves were picked, washed in distilled water air dried and weighed. The weight of the leaf was 0.1kg. The dried leaves were pounded to powdered form using a mortar and pestle. The leaf powder was suspended in methanol for 24 hours before filtering with a white filter cloth and boiled for 30 minutes. The resulting methanol extract was allowed to attain room temperature and was evaporated to dryness using a water bath as a source of heat. The percentage yield of the extract was 6.41% (w/v). The pasty residue was used to prepare a standardized solution of the leaf extract in calculated amount of distilled water with standardized concentration of 0.46 g/ml. The standardized solution (the stock solution) was stored airtight in plastic bottles and kept frozen.

2.1.2 LABORATORY ANIMALS

Exactly twenty four male albino rats weighing 148 ± 30g obtained from the Animal Holding Unit of the Institute of Agricultural Research and Training (IART) Apata-Ibadan, Nigeria were used for the study. The rats were fed with Guinea grower mash (product of bendel feeds and flour mills Ltd, Ewu, Edo state, Nigeria). The rats were grouped into four (4) groups, each groups consisting of six (6) rats.

2.2   INSTRUMENTS USED

The instruments used for this analysis include;

Beam weigh balance

Electronic weigh balance

 

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